Linoleate and α-linolenate synthesis by isolated spinach (Spinacia oleracea) chloroplasts

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Linoleate and alpha-linolenate synthesis by isolated spinach (Spinacia oleracea) chloroplasts.

Diacylgalactosylglycerol synthesis was a prerequisite for the incorporation of [1-14C]-acetate into linoleate and alpha-linolenate of isolated spinach (Spinacia oleracea) chloroplasts. Oleate at position 1 of diacylgalactosylglycerol was desaturated to linoleate and alpha-linolenate both in the light and in the dark. Some desaturation of palmitate was also observed after prolonged incubations.

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On the control of long-chain-fatty acid synthesis in isolated intact spinach (Spinacia oleracea) chloroplasts.

1. Chloroplasts isolated from spinach leaves by using the low-ionic-strength buffers of Nakatani & Barber [(1977) Biochim. Biophys. Acta.461, 510-512] had higher rates of HCO(3) (-)-dependent oxygen evolution (up to 369mumol/h per mg of chlorophyll) and higher rates of [1-(14)C]acetate incorporation into long-chain fatty acids (up to 1500nmol/h per mg of chlorophyll) than chloroplasts isolated ...

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Circular Dna Molecules Associated with Chloroplasts of Spinach, Spinacia Oleracea

Results of cytochemical studies (Sagan and Scher, 1961 ; Ris and Plaut, 1962 ; Kislev et al ., 1965) and of studies involving cell fractionation and buoyant density analyses (Sager and Ishida, 1963 ; Ray and Hanawalt, 1964 ; Brawerman and Eisenstadt, 1964 ; Edelman et al ., 1964 ; Wells and Birnstiel, 1969) have shown that chloroplasts of microorganisms and of higher plants contain DNA which is...

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Novel defensin subfamily from spinach (Spinacia oleracea).

Antimicrobial peptides (So-D1-7) were isolated from a crude cell wall preparation from spinach leaves (Spinacia oleracea cv. Matador) and, judged from their amino acid sequences, six of them (So-D2-7) represented a novel structural subfamily of plant defensins (group IV). Group-IV defensins were also functionally distinct from those of groups I-III. They were active at concentrations < 20 micro...

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Activity of phosphatidylcholine-transfer protein from spinach (Spinacia oleracea) leaves with mitochondria and chloroplasts.

A low-molecular-weight protein catalysing the transfer of phosphatidylcholine from liposomes to mitochondria and chloroplasts has been isolated from spinach (Spinacia oleracea) by chromatography on Sephadex G-75.

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1979

ISSN: 0264-6021

DOI: 10.1042/bj1840571